Guideline for the application of heart rate and heart rate variability in occupational medicine and occupational health science

This updated guideline replaces the “Guideline for the application of heart rate and heart rate variability in occupational medicine and occupational health science” first published in 2014. Based on the older version of the guideline, the authors have reviewed and evaluated the findings on the use of heart rate (HR) and heart rate variability (HRV) that have been published in the meantime and incorporated them into a new version of this guideline. This guideline was developed for application in clinical practice and research purposes in the fields of occupational medicine and occupational science to complement evaluation procedures with respect to exposure and risk assessment at the workplace by the use of objective physiological workload indicators. In addition, HRV is also suitable for assessing the state of health and for monitoring the progress of illnesses and preventive medical measures. It gives an overview of factors influencing the regulation of the HR and HRV at rest and during work. It further illustrates methods for measuring and analyzing these parameters under standardized laboratory and real workload conditions, areas of application as well as the quality control procedures to be followed during the recording and evaluation of HR and HRV. Supplementary Information The online version contains supplementary material available at 10.1186/s12995-024-00414-9.

• Statement on the usability of mobile wearables (Section: Technical possibilities and requirements), • Discriminatory power between HRV and pulse wave variability (Section: Technical possibilities and requirements), • Addition of innovations in the field of measurement technology since 2014 (Table 1), • Updating the overview table of HRV parameters (Appendix 1), • Updating the evidence on factors influencing HRV and restructuring (Table 2),

Introduction
The HR provides information about the strain of the cardiovascular system in response to physical and mental workload.The HRV gives additional information regarding the dynamics and mechanisms of cardiovascular regulation [1].Both physiological parameters have been established for the use in inpatient and outpatient care (e.g.cardiology, intensive care, endocrinology, neurology, occupational medicine, sports medicine, obstetrics) as well as medicine and scientific research (occupational physiology, exercise physiology, occupational science, sport science, psychology and pharmacology) for many years because of their non-invasive data acquisition and comfortable methods of, analysis.

Definitions
In medical assessment, a distinction is required between the HR (measured centrally) and the pulse rate (measured peripherally).A difference can occur, for example, in certain forms of cardiac arrhythmia as a pulse deficit.HR is defined as the number of beats or contractions of the heart per minute.It can also be calculated as a ratio of 60,000 and the average NN interval 1

in milliseconds.
The HR is a measure of the individual workload response of the cardiovascular system and is affected by various factors (see Section: Factors influencing the individual HR and HRV).HR should be distinguished from the pulse rate, which is defined as the number of pulsations per minutes palpated at the periphery, e.g. at the wrist or at the neck.A difference between HR and pulse rate may occur in certain types of cardiac arrhythmias where some contractions of the heart do not produce a palpable pulse at the periphery.A difference between the HR and the pulse rate is called pulse deficit.
In adults, the resting HR (HRrest) is usually between 60 and 80 beats/min (bpm).In people with endurance training, values well below 50 bpm may be obtained at rest The HR rest typically varies between 60 and 80 bpm in adults.It is usually higher in children i.e. up to 120 bpm [2].In endurance trained adults, the HR rest is often below 50 bpm.
The maximum achievable HR varies greatly between individuals and depends on age and biological sex, among other factors.It is recommended to determine it individually as part of ergometric exercise during general dynamic muscle work.The use of formulae to estimate the maximum heart rate (HR max ) should be used with great caution due to the very wide variation The HR reaches a maximum during physical exertion.The maximum value differs between individuals and decreases with age.The most commonly used empirical formula for estimating the HR max is [3]: However, this formula underestimates the HR max in individuals > 40 years of age [4].Based on a meta-analysis and their own examinations, Tanaka et al. [4] calculated a regression formula to estimate age-dependent HR max by: in which sex-related differences have not been considered [4,5].The high inter-individual heterogeneity of the HR max as a function of age is confirmed by clinical studies for women [6] and men [7].The determination of the individual HR max requires maximum physical exertion under conditions of dynamic muscle activity of a larger muscle mass, e.g. a cardiac stress test using treadmill or bicycle ergometry [8].Depending on the specificity of the subjects, usually other instruments like the arm crank ergometer may be used as well [9].
The recovery HR (HRrecovery), the working HR (HRwork) and the integral of the HRrecoveryare available for further assessment of the performance of the cardiovascular system In the field of exercise physiology, the HR following a maximal exercise test is frequently taken as an indicator of the fitness level of a subject.The value is measured one minute 2 after the cessation of a maximal exercise test.It reflects the rapid regulative phase of recovery and is called the HR recovery .
In the fields of occupational medicine and occupational health science, the HR during work 3 (HR work ) is taken into consideration while analysing the respective activity, e.g., evaluation of physical work.The HR work is defined as the difference between HR rest and the value measured during physical work [10][11][12].HR work is also known as net HR (HR net ) [13].HR net correlates better with the physical HR max = 220 − age HR max = 208 − 0.7 × age 1 NN interval = normal-to-normal interval, used synonymously with the terms RR interval, IBI (interbeat interval), cycle length variability, heart period variability. 2In some load protocols, the HR is also analysed after 3-5 min after the end of the load. 3Older publications often use the term work pulse rather than work HR.exertion than the HR, provided a resting phase without physical or emotional stress of at least five minutes (ideally, fifteen minutes) before starting the work is maintained to assess a valid baseline HR.This is a necessary requirement while carrying out tests in the laboratory, whereas in cases of measurements at real workplaces, it could be difficult to achieve these conditions before the working shift.Under circumstances of unreliable and not representative HR rest measurements, absolute HR might better reflect the intensity of the workload during physical exertion than HR work .Alternatively, the reference HR (HR reference ) can also be determined for light dynamic work (see Section Heart rate at rest).
In addition, the individual physical exertion is also frequently described by calculating the summated recovery HR as a measure of the fatigue and recovery [14].For this procedure, all heart beats during the recovery phase are summed until HR reaches the baseline level (e.g., HR rest ) (Calculation of the integral of the heartbeats above HR rest over the time from end of exercise to normalisation of HR).
HRV is based on a mathematical analysis of a time series of consecutive heart actions -the so-called NN intervals.
The term HRV comprises a number of mathematically calculated parameters, which characterise the variance, rhythm or complexity of a time series of consecutive heart beats -the so-called NN interval.Because of robustness and reliability issues the R-wave is usually used as a sign of electrical heart activation during automatic detection (Fig. 1).A detailed list of the frequently used HRV parameters can be found in Section: Analytical methods and parameters of HRV.

Physiological mechanisms of HR
The autonomic modulation of HR by sympathetic cardiac nerves and the vagus nerve (parasympathetic) is primarily mediated via the sinus node.
During the resting phase, the frequency of the heartbeat is triggered by the primary impulse generating tissue (pacemaker), the sino-atrial node (SA-node).The frequency of the non-innervated sinus node is stated differently in the literature.In the short term after heart transplantation, in which the transplant is denervated by the surgery, the HR is higher than the normal physiological HR, which is 60 to 80 bpm [15].For a longer period of time after transplantation, it changes back towards normal physiological HR, probably due to partial reinnervation.After transplantation, however, the beta-adrenergic activation of the sinus node cells by circulating adrenaline from the adrenal medulla continues, which may contribute to the increased HR rest .All downstream pacemaker tissues also capable of spontaneous depolarisation (AV node, bundle of His, Purkinje fibres) exhibit lower activation frequencies.The autonomic modulation of HR by the sympathetic and the parasympathetic (on the heart singularly influenced by the vagus nerve) is primarily mediated by the SA-node.This two-way control of the autonomic nervous system (ANS) was demonstrated in blocking experiments of the adrenergic (beta-blockers) and muscarinic-cholinergic (atropine) receptors [16][17][18].

Physiological mechanisms of HRV
The HR is subject to physiological variability even during constant stress, which reflects the interaction of the sympathetic nervous system (SNS) and vagus nerve (as part of the parasympathetic nervous system [PNS]), among other factors.
Even under constant physical exertion, HR shows a physiological variability, which predominantly reflects the interplay in the ANS between the SNS and the N. vagus (as part of the PNS).The sympathetic part of the ANS leads to a reduced HRV through the release of adrenaline and noradrenaline, while the parasympathetic (vagal) part leads to an increase in HRV through the release of acetylcholine [19].
At rest and during mild exertion, the parasympathetic (vagal) control outweighs the sympathetic effect.This leads to an increased variability of the heartbeats: the difference in the gap between two consecutive heartbeats increases.The HRV analysis is used particularly for the differential evaluation of the interplay between the sympathetic and the PNS under various conditions.Therefore, the quantification of the autonomic activity is carried out by analysing the periodic fluctuations of the heartbeat.Rapid changes in the HR with a cycle length of about 2-7 s are closely associated with breathing (Respiratory Sinus Arrhythmia [RSA]).These high-frequency fluctuations are modulated almost exclusively by the parasympathetic branch of the ANS (vagus nerve); whereas the slow fluctuations (cycle length of about 10 s) are modulated by the efferent nerve fibers of both parts of the ANS [20].However, for the interpretation of HR and HRV, it must be taken into consideration that both parameters reflect the net effect of autonomic cardiac efferent nerve activity but also other modulating factors like humoral and mechanical influences during physical exertion, heat and other environmental factors.In the case of temperature changes, the effects are mediated on the one hand via the modulation of the ANS and on the other hand directly via temperature effects on the sinoatrial node cells of the heart.
The vagal resting tone is higher the better the heart is adapted to cope with high physical stress, which is why trained people (e.g.endurance athletes) generally have a higher HRV in addition to a lower HR rest .In addition to changes in the activity of autonomic efferent nerve fibers, endurance training also leads to changes in the expression of ion channel proteins and membrane transport proteins [21], which has an additional lowering effect on HRV.

Technical possibilities and requirements
Different measuring systems are available for recording heart actions.Their measurement accuracy for a subsequent HRV analysis varies.It is recommended to use an ECG-based measurement for this purpose.The devices should measure non-invasively at a high sampling rate (ideally 1,000 Hz, minimum 250 Hz), be mechanically robust and non-reactive.
Several methods are available to record the interbeat intervals: stationary ECG instrument -which is more suitable for laboratory studies or intensive care units -and mobile measurement techniques that are convenient in field studies.The mobile measuring systems include 24-hour ECG devices, chest strap systems with direct storage or storage on an external data module (e.g. in a separate heart rate monitor) as well as a number of mobile measuring systems that have become available on the market in recent years (e.g.watches without a chest strap, systems with measurements in the ear canal, etc.).What all measuring systems have in common is that they record the NN intervals with different levels of measurement accuracy and deviations can therefore occur in the subsequent HRV analysis [22,23].
Pulse oximeters can also determine the distance between two pulse waves.However, these differ from the NN intervals measured on the heart using electrodes.A systematic review [24] showed that only in young, healthy subjects under resting conditions are there acceptable correspondences between NN intervals and pulse NN intervals, but HRV parameters based on the pulse NN intervals are sometimes significantly increased (e.g. in HF).It is therefore important to make a strict distinction between HRV and the so-called pulse rate variability (PRV).
For HRV analysis, a so-called "beat-to-beat recording" with assessment of all cardiac actions and a high sampling rate (ideally 1,000 Hz, minimum 250 Hz [25]) is gold standard in order to record the distances between individual cardiac actions with high temporal accuracy.
In addition, the instruments should fulfill the following requirements: • non-invasive, • mechanically robust (for examinations at workplaces, which involve heavy physical work or difficult environmental factors like heat, cold and wet conditions) and.• non-interfering (the method itself should not influence the results in any way).
The advantages and disadvantages of the different measurement systems are given in Table 1.

Electrodes
It is recommended that the electrodes be adequately prepared for optimal measurement results.
The following should be done to avoid errors during measurement: • adhesive electrodes should be used so that they do not lose contact with the skin even after longer periods of recording (e.g.24 h) and in cases of sweating, • the electrodes on the chest belt (contact points) should be moistened, • the chest belt should fit firmly and.
• a textile strap should be preferred, because it can adapt itself optimally to the individuals upper body.

Preparation of the skin
It is recommended that the skin be adequately prepared for optimal measurement results.This includes, among other things, reducing the oil film on the skin and, if necessary, removing existing (chest) hair.The skin should be prepared carefully in order to obtain optimal results of measurements, especially if long-term recordings (24 h) are carried out.In cases of skin-electrode contact with high impedance, the quality of the recording decreases and the probability of the appearance of artifacts are high.
The main objective of preparing the skin is to remove the natural oil film of the skin.This reduces the contact resistance between the skin and electrodes and enables a better adherence of the electrodes.The contact points on the skin for the electrodes are first cleaned with a dermatologically safe, degreasing solution (e.g.alcohol solution).However, any damage or injury to the skin has to be avoided.If chest hair grows, it may be necessary to carefully remove the hair from the corresponding adhesive areas before attaching the adhesive electrodes.An additional fixing of the electrodes and the cables is useful for long-term recordings or other conditions.

Lead choice and electrode positioning
It is recommended to select the ECG lead with the largest amplitude of the R wave of the QRS complex.
The ECG leads must be chosen based on the largest amplitude of the R-wave of the QRS complex (see Fig. 1).In principle, recordings from a single lead are sufficient.If possible, multiple leads should be used to enable a reliable correction of artifacts.
During the automatic determination of the NN interval, it should be ensured that R-wave detection is consistently based on the same lead.Changing the lead during the same recording can lead to an artificially generated increase in the HRV.While the point of time at which the QRS complex begins is almost identical in most of the leads, the fiducial point (R-wave), which serves as the basis for determining the NN interval, can significantly vary between the different leads [26,27].
The positioning of the electrodes influences the quality of the recordings.If electrodes are not positioned appropriately, recording quality might suffer, resulting in an accumulation of artifacts.The intercostal spaces are suitable areas for positioning the electrodes.Within these spaces, flat and even areas of the skin should be selected (e.g.positioning above dermal naevi should be avoided).

Quality assurance while determining the HR
When recording the HR, quality assurance requires the determination of HR rest , artifact control, the highest possible sampling rate and the consideration of possible influencing factors.
The following aspects should be taken into consideration for the purpose of quality assurance: • the determination of HR rest4 before the beginning of the exertion as physiological baseline for the evaluation (see Section: Heart rate at rest), • checking for artifacts and, if possible, removal of artifacts (e.g. by visually checking the data during analysis, automatic methods for correcting artifacts), • a high sampling rate (see above), • the possible influencing factors depending on the case (see Table 2) and.• the circadian rhythm should be kept in mind for comparable examinations.

Quality assurance while determining the HRV
For quality assurance when determining HRV, it is recommended to take a resting ECG, check for artifacts and, if necessary, correct them, use recordings with only a few extrasystoles and choose a suitable analysis method and measurement duration, if possible, to use high sampling rates and to take possible influencing factors into account.
The following aspects should be taken into consideration for the purpose of quality assurance: • a resting ECG should be recorded before the HRV is analysed in order to rule out cardiac arrhythmias (e.g.atrial fibrillation), • recordings with more than 1% of ventricular or supraventricular extrasystoles should be evaluated critically because of the apparent increase in the HRV [203], • checking for artifacts and, if possible, removal of artifacts (e.g. by visually checking the data during analysis, automatic methods for correcting artifacts), • the analytical method of choice (e.g.Fast Fourier Transformation, Autoregressive Model, Trigonometric Regressive Spectral Analysis) to enable comparable interpretations (see Section: Analytical methods and parameters of HRV), • the selected duration of recording (subsequent length of the sequence of analysis) or the underlying amount of data depending on the analytical method selected and the research question (see Appendix 1), • a high sampling rate (see above), • the possible influencing factors depending on the analytical method selected and the research question (see Table 2) and.• the circadian rhythm should be considered as a possible confounder if comparing repeated measurements.
In the case of short-term recordings, the selection of a suitable, representative area of the NN intervals is an important quality criterium for HRV analysis.For this, the non-steady setting phase at the beginning of the examination and the recordings with artifacts should be avoided for the analysis as far as possible (see Fig. 2).

Other sources of interference
There are also other sources of interference (such as electromagnetic fields) that can influence the recording of HR and HRV.
When chest belts with wireless data transmission are used in the vicinity of electromagnetic fields from power poles or power supply lines [1] or used in vehicles and their vicinity [204,205], interferences can occur.Artifacts due to body movements and due to electrical activity of other muscles can occur during physical activity.In the case of an ECG recording, these artifacts should be detected and manually removed at the end of the recording, whereas in cases of gathering data without ECG recording (like in most cases of chest belts systems), it is not always possible to attribute the artifacts to the movements.

Analytical methods and parameters of HRV
HRV can be quantified using time and frequency domain methods as well as nonlinear analysis.Care must be taken to make the correct selection in relation to the objective and evaluation time.

Table 2
Factors influencing HR and HRV, sorted according to the four main areas depicted in Fig. 4, sorted alphabetically within the main area

Circadian rhythm/time of the day
The HR follows a circadian rhythm, with a fall of HR at night [47].
The HR follows a circadian rhythm, but the HRV is decreased at night due to the predominance of the parasympathetic activity and reduced during the day because of the predominance of the sympathetic activity [48].

Genetic
HRV appears to vary between members of different ethnic backgrounds [49].

Pregnancy
During pregnancy there is usually an increase in HR [50].
During pregnancy, a reduction in HRV usually occurs as the pregnancy progresses [50] and is lowest in the 2nd trimester [51,52].

Biological sex
The HR is normally higher in women than in men [53].
Some of the studies showed a higher baseline sympathetic activity in women [36,37,60,61].

Cancer diseases
The influence of breast cancer on HRV is unclear [62].

Cardiovascular diseases
Cardiac insufficiency leads to a raised HR [63] and unrestricted maximum HR.
In patients with previous myocardial infarction, the activation of the sympathetic nervous system often leads to an increase in the HR, which is important for the prognosis [70][71][72][73][74][75].
HRV is usually reduced in coronary heart disease (CHD) with and without angina pectoris and after myocardial infarction [76,77].

Chronic renal insufficiency
In chronic kidney failure, HRV is usually reduced [78].

Duchenne muscular dystrophy
HRV is usually significantly reduced in the early stages of Duchenne muscular dystrophy and in manifest disease [80].

Metabolic disorders
Diabetes mellitus is often associated with increased sympathetic activity and hence a raised HR [83].
The HRV is often reduced in patients with diabetes mellitus [58,[84][85][86][87], however, a correlation between the value of the HRV and the duration of the diabetes exists especially in cases of badly controlled diabetes [88].
The reduction is due to peripheral neuropathy due to microcirculation disorders [89].

Pain
In chronic pain, HRV is usually reduced [98].

Influencing factor
Effect on resting heart rate (HR rest )

Effect on heart rate variability (HRV)
Psychiatric disorders Patients with anxiety disorders and panic attacks usually have an increased HR [99].
Patients with anorexia nervosa usually have a reduced HR [104].
HRV is usually reduced in patients with anorexia nervosa [102].
HRV is usually reduced in patients with bulimia nervosa [105].
Posttraumatic stress disorder often leads to a reduced HRV [106].
In the case of substance addiction [38], HRV is usually reduced.

Rheumatoid arthritis
Based on a systematic literature search, HRV does not currently appear to be changed in the presence of rheumatoid arthritis [120].

Sleep disorders
A reduction in HRV in the presence of sleep disorders is currently not supported by the scientific literature [121].Something similar can be found in untreated obstructive sleep apnea syndrome.

Stroke
A stroke is usually associated with reduced HRV [122].

Alcohol consumption
With acute alcohol consumption, HRV is usually reduced [123].Low, constant alcohol consumption with an alcohol content of one standard drink for women or two standard drinks for men usually leads to a shortterm but no long-term change in HRV or an increased HRV, while chronic alcohol abuse leads to a reduction of HRV [123,124].
Body fat/body weight Increased body mass index (BMI) generally leads to a raised HR [125], which can be partly explained by the stimulating effect of leptin on central sympathetic neurons [126,127].
Increased body mass index (BMI) and increased mass of body fat often cause a fall in the HRV [128]. in HRV in subjects with chronic illnesses [132].
Initially, there is a rise in the resting HR due to the increased physical activity; however, regular exercise without symptoms of overtraining leads to a decrease in the HR due to an increase in the parasympathetic activity and an optimisation of the cardiac output [133].The expression of ion channels for the pacemaker potential is reduced [21].Therefore, endurance training often results in exercise-induced bradycardia [134][135][136][137].
Initially, there is a fall in the HRV due to increased activity of the sympathetic system as a result of the physical activity [138], but regular physical activity leads to an increase in the parasympathetic activity which in turn causes a rise in HRV [36,131,[138][139][140]. Endurance training normally increases the HRV [130,131,[141][142][143].These effects can be also seen in patients with myocardial infarction and patients with cardiac insufficiency [141] or Diabetes mellitus II [144].
Smoking Active [145] and passive smoking [146] can lead to an increase in HR.
Smoking can lead to a decrease in HRV [147], this effect is dose dependent [146].Even in non-smokers, passive smoking e.g. at home or at work leads to a reduction in the HRV [146][147][148].

Breathing
During inspiration there is a short-term increase in HR, during expiration there is a short-term decrease in HR [158,159].This is essentially due to pulmonary afferents from stretch receptors and interactions from central respiratory neurons to the circulatory center in the medulla oblongata.The effects of respiration on HRV are reflected in the form of respiratory sinus arrhythmia (RSA) and is seen in the HF band.On the whole, the HRV parameter, RMSSD, does not seem to be affected by respiration [160].
For the rest of the parameters, the present state of knowledge is not conclusive [161][162][163].

Cold, low temperatures
In men, low ambient temperatures usually lead to a decrease in HR both at rest and during exercise, while in women there is no decrease in HR, but rather a slight increase in HR [164].
Only few studies about the effects of low temperatures on HRV are currently available: a reduction in the sympathetic activity and thus a raised HRV has been observed [165], while long-term exposure to cold, including in winter months or occupational exposure to cold, no influence on HRV could be shown [166][167][168].

Hazardous substances
Neurotoxic substances can lead to a reduction in the HRV: e.g.carbon disulphide [169,170], however, not in the case of long-term low-dose exposure [171]; acute diesel and biodiesel inhalation [172]; chronic lead [173,174], acute cadmium [175] or long-term mercury exposures [176] and neurotoxic styrene exposure [177,178].The data regarding the effects of chronic solvent exposure is not conclusive, both -a fall in the HRV and no differences -have been described [179][180][181].
In contrast, there was no evidence of a reduction in HRV through mercury exposure [182].Only fetal mercury exposure appears to lead to a reduction in HRV [176].

Influencing factor
Effect on resting heart rate (HR rest )

Effect on heart rate variability (HRV)
Heat, high temperatures High environmental temperatures lead to an increase in the HR [13,184,185] caused on the one hand by direct temperature effects on the sinoatrial node and on the other hand by the increase in sympathetic activity as a result of the activation of warm receptors.
High environmental temperatures lead to an increase in the sympathetic activity and a reduced HRV [166,186].

Hypoxia
Hypobaric hypoxia usually leads to short-term sympathetic activation [187] and long-term to a reduction in HRV [188].

Noise
Noise often causes a rise in the HR [189], caused by activation of sympathetic nerves [190].
Only few studies that give information about the effects of noise on HRV are available; HRV appears to fall in the presence of noise [191][192][193][194].

Pharmacological drugs
Pharmacological drugs can have an increasing or decreasing effect on HR [195].
Pharmacological drugs can have an increasing or decreasing effect on HRV [195].

Shift work including night shift
Shift work with a night shift usually results in an activation of the SNS and a reduction in the PNS and thus a reduction in HRV, whereby there is a correlation between the duration of shift work in years and the reduction in HRV [196][197][198][199][200][201][202].
HRV is quantified using time and frequency domain methods as well as methods of non-linear analysis (see Fig. 3).
Time domain methods are divided into statistical and geometrical methods.In the case of the statistical methods, the NN intervals are evaluated mathematically with respect to its variance and the measurement of the rhythm is tagged with the time dimension or the percentage values, whereas geometrical methods provide an evaluation of HRV based on geometric forms.For these purpose histograms, HRV triangular index and its modifications, triangular interpolation of the NN interval histogram are used [206].
The analyses of the frequency range include, among others, the Fast Fourier transformation and Autoregression methods [207,208].Spectral analysis decomposes the periodic oscillation of the NN-signal, into different frequencies and amplitudes.This makes it possible to split the NN interval series and the periodic oscillations of the heartbeat into different frequencies and amplitudes, which in turn represent different physiological processes or different control systems [209].
In some cases, the Lomb algorithm is also used to analyse recordings with varying lengths and non-equidistant sampling.This represents a spectral analysis method of non-equidistantly sampled measured values [210].The Lomb algorithm is an extremely slow method; but approximation methods have been established to speed up the application of the algorithm [211].
The methods of non-linear dynamics (e.g.Approximate Entropy [ApEn], Sample Entropy [SampEn], Detrended Fluctuation Analysis [DFA]) [30,[212][213][214] vary from the traditional time and frequency parameters in that they do not reflect the strength of the HRV, but they rather indicate qualitative aspects of the series of NN intervals [212].These methods often prove to be suitable for longterm as well as short-term recordings and are considered more robust against artifacts.
One form of visualisation of the time series of NN intervals is offered by the so-called Poincaré Plot5 [213] (see Fig. 3).From this plot various indices can be determined and interpreted (e.g.length and width of the scatter-plot).Further, the form can also give hints about certain diseases [215].
A detailed listing of the HRV parameters is given in Appendix 1.Aside from acute physical exercise/exertion HR and HRV can be affected by several modifiable and non-modifiable factors.In addition to physiological parameters that cannot be influenced (e.g.age), there are a number of changeable influencing factors -e.g.living habits of the test subjects or the resulting consequences or external conditions.Furthermore, a variety of diseases are associated with reduced HRV, although the influence on the ANS can be viewed as a consequence of the disease and only rarely as a potential cause.
The individual influencing factors can be divided into four main areas (physiological factors that cannot be influenced, diseases, lifestyle factors that can be influenced and external factors) (see Fig. 4).The most relevant factors for investigations in the field of occupational medicine and occupational health science are described in Table 2.The consideration of these factors is of importance when HR and HRV are evaluated.In addition, various other factors and conditions (e.g.HRV in patients with sepsis that needs intensive care) have been mentioned in scientific literature.As these cases are normally not relevant in the field of occupational medicine and occupational health science they will not be considered any further in the current guideline.
Pharmacological drugs can have significant impact on the ANS or the electrical conduction system of the heart and thus should be considered when assessing and evaluating HR and HRV.Due to the large number of possible pharmacological interactions, the groups of beta blockers, acetylcholinesterase inhibitors, antiarrhythmics and psychotropic drugs are only mentioned here as examples [195].

Evaluation and interpretation of HR and HRV
In order to be able to correctly evaluate and interpret the results of HR and HRV analyses, it is recommended that the specific question, the data collection method used and the evaluation strategy be coordinated in advance of the examinations.
For a valid and reliable evaluation and interpretation of HR and HRV adequate study designs, data sampling strategies and analysis methods are necessary prerequisites.HR and HRV parameters mirror the individual  2).Thus, measurements of HR and HRV should always be combined with complementary data (e.g., questionnaire about the subjective stress, perception of stress and the state of health).If possible, information about the ambient conditions at the workplace, like noise and temperature, should be collected at the same time.

Heart rate (HR)
The main influencing factors for HR are dynamic loads on large muscle groups, but also static muscle loads, thermal and psychological loads.
Important factors that influence HR are dynamic activity of larger muscles, static muscular load of smaller muscles and thermal stress as well as mental workload [217,218].These factors often act together on the cardiovascular system and can induce a corresponding increase of HR during exertion.These effects were studied by e.g.Hettinger and Wobbe [189] in cases of different muscle, temperature and thermal radiation loads.A demarcation of the individual components is possible under controlled conditions only.So, HR during dynamic work of larger muscles can be used for estimating the energy expenditure only if the activity of smaller muscles and the mental workload are negligible and thermal conditions remain neutral [218].Additional, static muscle strain as well as psychological and thermal strain lead to a decrease in the quality of the correlation between energy expenditure and HR (weak correlation coefficient).

It is recommended to use HR rest as the baseline value for interpreting HR changes under workloads
The HR rest is the preferred baseline value for an individual evaluation of the HR during physical exertion (see Section: Quality assurance while determining the HR).Baseline measurement conditions (e.g.posture of the person, duration of recording) should be standardized to enable within-and between-subject comparisons.Both, an increased or a decreased HR rest can be associated with an apparent cardiac disease [219,220].After considering physiological contributors to HR rest (see Section: Factors influencing the individual HR and HRV) persons with unexplained higher (tachycardia) or a lower (bradycardia) HR rest should be subjected to a cardiological examination.
Sometimes the determination of HR rest is difficult in field studies, due to confounding effects on HR (psychological factors, environmental conditions like noise, ambient temperature etc.).Therefore, Hettinger and Wobbe [189] recommended the determination of a HR reference during Fig. 4 Grouping of the different factors influencing HRV into four main areas (modified taken from [216]).* = HRV reduction as a result of the physiological response to the physical stimulus.
light dynamic work (e.g.20 W on a bicycle ergometer for 10 min).Since this workload is typically perceived as a "light exertion", the effect of psycho-emotional stress ("psychological heart rate") is largely eliminated.Compared to the resting value in the supine position the HR increases by an average of 18.5 bpm in men during this procedure; while in women, an average increase of 24.5 bpm with relatively narrow limits of agreement can be expected [189].

Maximum heart rate (HR max )
It is recommended to use the HR max as an exercise criterion and to determine it using a standardized exercise protocol.
The HR max serves as a criterium for maximum physical exertion and can be determined during a standardised exhausting exercise protocol [221].The most widely used methods for this are the treadmill and the bicycle ergometry.An optimal motivation to bring about the maximum performance and the observance of the stop criteria are the main requirements for the determination of the HR max .Personal physical exertion can also be measured using the Borg scale as an example [222,223].However, one should keep in mind that apart from factors like age, sex and fitness level [224] and certain bradycardia producing drugs [8], the value of the HR max determined largely depends on the muscle mass that is used.
For an appropriate estimation of cardiac workload, the interpretation of the HR response during a given (occupational) physical task should always be referred to the individual HR rest and HR max (see Section: Physiological mechanisms).Here, a value of the HR during physical exertion (occupational), which lies closer to the HR max , indicates a higher degree of stress on the heart.The continuous performance limit can also be referred to for the interpretation (see Section: Continuous performance limit (CPL)).

Recovery heart rate (HR recovery )
HR recovery can be used to estimate the recovery ability of the cardiovascular and metabolic systems.
The HR recovery can be used to estimate the recovery capacity of the cardiovascular and the metabolic systems.It strongly correlates with the function of the parasympathetic branch of the ANS [225] and typically decreases exponentially after the end of the exertion.The main factors that influence the temporal kinetic of the recovery of the vagus are intensity, duration and method of the physical exertion, initial performance level and the type of recovery [226][227][228].

Continuous performance limit (CPL)
The CPL for physical work is the maximum physical work that can be maintained over a work shift (approx.8 h) without progressive symptoms of fatigue.
The CPL of physical exertion characterizes the maximum muscular work that can be maintained over a regular working shift (about 8 h) without any progressive symptoms of fatigue and where the measurable physiological parameters return to baseline or fall even below baseline within 15 min after the work cessation [13].If the CPL is adhered to, overloading and injuries can be avoided and adequate recovery for the next (work) shift is possible.The CPL can be used for the identification of muscular physical exertion without fatigue (below the CPL) and muscular exertion inducing fatigue (above the CPL) with respect to an 8-hour working shift [10,229,230].The value of CPL can be determined using cardiac (e.g., HR) as well as metabolic parameters (e.g., energy turnover, lactate).Spiroergometry can also be used as an alternative method for the determination (e.g.40% of the maximum oxygen intake).HR in particular is suitable as an easy-to-collect cardiac parameter for recording cardio-pulmonary stress.In the cases of dynamic activity of larger muscle masses, the CPL ranges between 105 and 110 bpm or alternatively between HR rest + 30-35 bpm [13].It should be noted that HR used for the determination of the CPL, like the individual HR rest and HR max, also underlies a strong individuality due to e.g., age and the level of physical fitness.
Below the CPL, the HR shows a linear increase along with the intensity of workload.In the case of light work with a constant performance over time, the HR reaches an almost constant deflection ("steady state") within a short period of time (few minutes).Typically, this "steady state" can be maintained over the entire 8-hour working shift (see Fig. 5).
Small, short-term overshoots beyond the CPL (e.g., HR of 130-140 bpm) are common during a work shift and do not pose any health risks, while scheduled breaks during constant physical exertions with a HR > 130 bpm help to overcome muscular fatigue.The more intense the physical strain is and the more the state of exhaustion is reached, i.e., the further the CPL is exceeded, the more these tiring activities must be limited with respect to duration.
If the CPL is being continuously exceeded, this kind of work is classified as heavy physical work or hard labour, in terms of energy [231].It leads to increasing muscle fatigue (along with anaerobic metabolism), which is generally reversible without any effects on health.There is a continuous rise in HR and a rise in fatigue as well (see Fig. 5).Heavy physical work is also relevant from the motor biomechanical point of view, because the skeletal system (joints, intervertebral discs) might be injured under relevant conditions [230].However, these aspects are beyond the scope of this guideline.
Apart from the CPL, the integral of HR recovery (see Fig. 5) is also considered an indicator of the individual physical exertion [14].
It should be noted that an evaluation of workload based on the CPL (as described above) is only valid, if larger muscle masses (> 1/6 of the total muscle mass) are dynamically active.If smaller muscles masses are used for dynamic tasks, the CPL has to be adjusted (e.g., decreased) in proportion to the muscle mass used.Typically, HR, HR work and oxygen update are lower compared to whole body exertion, despite similar or even shorter time until exhaustion.Sometimes, e.g. in cases of work done by the arm or hand, the CPL is not valid for estimating time to fatigue or exhaustion [232].Under these conditions local muscle load is limiting for endurance of work.In cases of isometric muscular work or with an increasing portion of isometric muscle activity during dynamic tasks, an evaluation of the workload intensity with respect to the CPL is not valid.
With respect to work structuring, the occupational tasks that are seen as being causal for an increase over the CPL should be considered in detail.

Heart rate variability (HRV)
In order to interpret HRV parameters under external stress, it is recommended that sensible planning and implementation of the measurement of cardiac actions be taken into account, especially with regard to the length of the recording.
Several methods are available for the analysis of HRV.In principle, the time domain parameters of HRV can be calculated using mathematical functions in established spreadsheet programmes.In addition, many manufacturers of mobile ECG instruments provide software programmes that enable the calculation of the time domain and the frequency domain parameters of HRV and also permit a non-linear analysis of the NN interval series.Freely available software packets with good documentation are also available.
For statements about the ANS (see Sections: Application to assess physical exertion and Application to assess mental workload), suitable HRV parameters should be used and recorded or analyzed with a time range suitable for the activity or rest measurement (see Appendix 1).It must be taken into account here whether an influence of the SNS or PNS is to be measured primarily and how long the recording time can reasonably be carried extended.While assumptions based on specific HRV parameters require long-term recordings (see Appendix 1), or the 24-hour measurement of HRV can be advantageous for gaining an overall impression of the ANS, HRV parameters that are suitable for short-term measurements are primarily used for the evaluation of physical stress at work with rapidly changing requirements.The first minute after a change in load should not be included in the data analysis, because transient adaptation processes of the vegetative control circuits dominate during this phase.
Due to the high inter-individual variability and numerous exogenous and endogenous factors affecting the NN interval, which usually cannot be changed as part of a measurement of the NN intervals, HRV analysis should be used only in combination with a baseline assessment or with repeated measurements during work under the comparable conditions.Some studies with reference values are available for the comparability of resting measurements.Attention must be paid to the age and biological sex dependence of HRV and to a reference population fitting to the test person.
In recent years, a number of studies has been published that use reference values to differentiate between increased and reduced HRV in individual HRV measurements.Due to the age and biological sex dependence of HRV described above, corresponding HRV reference values should also take this into account, but this has only been done in some of the published studies.An overview of studies with reference values can be found in Appendix 2. Furthermore, due to the possible genetic influence of ethnicity, a study should be used which was composed of test subjects who are similar to the working population.
Even though this provides limit values for HRV parameters, it should be borne in mind that these were calculated purely empirically.Therefore, no general health-related statements are possible.Standardised serial measurements (individual longitudinal studies) of HRV in combination with the medical history, clinical examination and other methods (e.g.questionnaires) can be valuable in explaining the individual health risks and help to evaluate the effectiveness of medical preventive measures.

Application in the fields of occupational medicine and occupational health science
In the field of occupational medicine and occupational health science, HR and HRV can be used to answer various questions.
The methods used for the recording and evaluation of HR and HRV can be used to gain an objective view of the activity of the ANS.The applications in the fields of occupational medicine and occupational health science may be summarized: • complementary examinations for the risk analysis and risk assessment to identify the core areas of work-related stress, • analysis of the individual physical and mental workload and a process-integrated measurement for an objective view of the workload response over the course of the working day, • determination of a health status indicator, • derivation of actions to be recommended for each individual e.g.workplace design, • determination of the fatigue and recovery behaviour and.• evaluation of interventions in medicine and occupational medicine.

Application to assess physical exertion
The use of HRV during physical stress, especially during dynamic muscle work, has long been established.The analysis of HRV also offers added value compared to the linear behaviour of HR due to the often two-phase or multi-phase behaviour under increasing loads.
The HRV parameters SDNN, RMSSD, Total Power, LF-Power and/or HF-Power should be used for this purpose.A minimum recording time of 5 min is recommended The evaluation of physical exertion using the HR especially during dynamic muscle work has been established for a long time.The knowledge gained through HRV in such cases and under standardised conditions are: (i) a proven correlation between HRV parameters and (ii) the metabolic and respiratory stress indicators, (iii) the multi-phase course during progressively increasing exertion and (iv) the recovery behaviour after varying degrees of exertion [233,234].This enables an accurate evaluation of the physical exertion without the use of a time-consuming, cost-intensive recording method that is also partly unavailable in the ambulatory and reactive forms.In addition to the parameters of total variability, e.g.SDNN or Total Power, the HRV parameters RMSSD, LF power and HF power and the non-linear indices are suitable for the determination of the acute physical exertion.These often show a two-phase or multi-phase behaviour under increasing load and, thus, have an added value compared to the linear behaviour of the HR, especially under moderate and high physical loads [235,236].Application to assess mental workload HR and HRV can be used for mental stress assessment.However, the selection of suitable HRV parameters is limited The HRV parameters RMSSD, LF, HF, LF/HF, DQ and SD1 should be used for this purpose.A minimum recording time of 5 min is recommended Mental stress is characterised by deflections in HR and HRV, which can therefore be used as mental stress indicators.Since the construct of mental workload is difficult to measure, HR and HRV are taken as parameters of general activation and can be used to reflect the vegetative balance of the organism.These parameters may therefore be used to draw conclusions about mental stress [101,[237][238][239][240][241][242][243][244][245][246][247][248].In addition, HRV can also be used as an indicator of both -the psychophysical condition of the organism and the restriction in the adaptability for biopsychosocial problems.The HRV parameters, RMSSD, LF, HF or LFnu and HFnu, LF/HF as well as DQ and SD1 are considered mental workload indicators.However, ULF and VLF are not suitable.Resting HRV may not be a predictor of cognitive capacity in cross-sectional studies.

Application for risk stratification of diseases
A reduced HRV correlates with increased morbidity and mortality in some diseases (e.g. after myocardial infarction, coronary bypass surgery, heart failure, stroke, chronic obstructive pulmonary disease, and high blood pressure) A prognostic value of HRV has currently only been proven for a few diseases.Large cohort studies have shown, among other things, that mortality was significantly higher in patients after myocardial infarction with a lower HRV compared to patients with higher HRV parameters in the post-infarction phase [77,249,250].According to a meta-analysis of five studies with a total of 3,489 patients, the mortality risk in patients after myocardial infarction and an SDNN < 70 ms was 21.7%, while for n SDNN > 70 ms it was 8.1% [249].The threeyears mortality rate after myocardial infarction was thus 2-3 times higher in the group with low HRV.
A correlation between reduced HRV (here SDNN < 93 ms) and recurrence of a coronary event was also found in patients after coronary bypass surgery.During an average three-year follow-up period, 13 out of 74 patients with reduced HRV suffered such an event, while this only affected 3 out of 132 patients in the comparison group (SDNN ≥ 93 ms) [251].A correlation between low HRV and overall mortality or cardiac endpoints was also found for patients with heart failure [252] and in patients with strokes, the individual HRV after the stroke correlated with the long-term outcome [122].
Schmidt et al. [253] were able to show in intensive care patients with multiple organ failure that the logarithmic value of the frequency-related HRV parameter VLF (lnVLF) allows an estimate of mortality in the short-term prognosis (up to 60 days).A systematic review of the relationship between chronic obstructive pulmonary disease and HRV reported that the reduction in HRV correlates with the severity of COPD [78].Furthermore, there appears to be a correlation between a higher HRV and longer survival in the context of tumor disease [254,255].In addition, it has been shown that HRV is reduced in individuals diagnosed with type I diabetes mellitus even before clinical signs of autonomic dysfunction appear [256].
There also appears to exist a correlation between a lower HRV and later manifestation of hy-pertension.In a group of 2,061 subjects controlled for age, biological sex, ethnicity, current smoking status, diabetes mellitus and educational status, subjects in the lowest quartile of HRV showed a 2.44-fold increased risk of new manifestations of hypertension after three years [257].Based on data from the Framingham Heart Study (2,024 subjects), Singh et al. [258] were able to show an increased risk of developing hypertension with reduced HRV in men, but not in women.Schroeder et al. [259] studying 11,061 subjects, found in those individuals which belonged to the lowest quartile -in relation to the RMSSD value -a 1.36 higher risk of developing high blood pressure compared to individuals of the highest quartile.
Reduced HRV correlates with prognosis scores for the occurrence of cardiovascular events A significant correlation between the reduction of the HRV parameter RMSSD and various prognosis scores for the occurrence of coronary heart disease or stroke was demonstrated in a collective of 11,994 subjects from the Mannheim industrial cohort [260].This could be shown for the PROCAM score according to Assmann et al. [261], for the Coronary Heart Disease Framingham Score [262] and for the cardiovascular prognosis indicators SCORE, related to the risk of coronary heart disease (SCORE-CHD) and cardiovascular disease (SCORE-CVD) according to Conroy et al. [263].

Application to evaluate preventive measures
HRV can be used as part of the evaluation of prevention measures in compliance with the quality criteria.Repeated HRV measurements should be used for this purpose HRV has been established particularly as a useful parameter for the evaluation of preventive measures like stress reduction courses, dietary changes, judicious use of stimulants, changes in eating behaviour, sport activities including the preventive monitoring of overtraining syndromes [264,265] and measures to reduce weight in order to evaluate the success of the corresponding preventive or interventional measures in longitudinal comparisons [266].For example, a change in the sympathetic-parasympathetic balance and a higher parasympathetic baseline activity (e.g. raised SDNN or RMSSD, reduced LF/HF ratio) indicate efficacy of the preventive measures.

Application in biofeedback
HR and HRV parameters can be used to objectify relaxation effects in the context of biofeedback.Long-term effects could not be shown with HRV-based biofeedback methods HR and HRV have been used for biofeedback in cases of stress recovery and recently also in the treatment of posttraumatic stress disorder e.g. for an objective view on the effects of stress relaxation [156,[267][268][269][270].However, until now, only short-term effects of such interventions have been observed.It has not yet been possible to demonstrate a long-term effect [270].In addition, suitable and validated methods must be used to utilise the shortterm effects of HRV biofeedback methods.
With reference to the determination and the evaluation of HRV (see Chap. 4), it is inevitable that the biofeedback methods, which determine the HRV with the help of pulsoximeter or respiratory activity, cannot be seen as valid measurement methods to assess HRV and therefore cannot be recommended for HRV-based biofeedback.

Conclusions
The practicability of the HR and HRV analysis on a daily basis for field studies at workplaces has been proven.These analytical methods can be used with a goal-oriented approach for various tasks when the methodological requirements are met.Under these conditions, HR and HRV can be recommended for the use not only in research institutes, but also for practising by occupational physicians and company doctors.This might help to improve diagnostic efficiency and to elucidate heart and health related mechanisms in the field of modern occupational medicine facing an ever-changing working environment and a demographic change in general.For a practical use a checklist is attached in the appendix to support scientists and users.Short-term variability [40] No clear assignment n.o no unit assigned. 1LF nu and HF nu behave reciprocally to each other, the sum of both parameters equals 100%.

Fig. 1
Fig. 1 Principle of determining the NN intervals from the ECG as a measure of the distance between two R-spikes

Fig. 2
Fig. 2 Principle of selecting a suitable 5-min range of the NN measurement from an artifact-superimposed recording with an early non-stationary range

Fig. 3
Fig. 3 Overview of the possibilities of HRV analysis with examples of possible graphical representations

Fig. 5
Fig.5 Heartrate during different loads (below or above the CPL as well as in the range of the CPL) with respective recoveries, schematic representation, modified according to Müller[14], CPL = continuous performance limit

Table 1
Advantages and disadvantages of the different measurement systems

Table 2 (continued) Influencing factor Effect on resting heart rate (HR rest ) Effect on heart rate variability (HRV)
Sammito et al.Journal of Occupational Medicine and Toxicology (2024) 19:15